一、个人简介

职称：讲师

学位：生物化工 工学博士

导师：硕士研究生导师

籍贯：江西吉安

二、学习和工作经历

学习经历：

2017.09-2023.01，华东理工大学，生物化工，博士研究生

2014.09-2017.06，江西师范大学，生物化工，硕士研究生

2009.09-2013.06，江西师范大学，生物工程，大学本科

工作经历：

2023.04-至今，江西师范大学，健康学院

三、研究方向

蛋白质理性设计、酶法不对称合成手性药物中间体的研究、新酶的挖掘与分子机理的研究

四、主持的项目

1. 国家自然科学基金委员会, 青年科学基金项目, 22308137, 氨基供体驱动ω-转氨酶合成手性胺的适应性催化机制及理性设计研究, 2024-01-01至2026-12-31, 30万元, 在研, 主持。

2. 江西省自然科学基金资助项目，青年基金项目，20232BAB213041，ω-转氨酶底物耐受性构效机制及理性设计研究，10万元，2023-07-01至2026-06-30, 在研，主持。

3. 江西师范大学新进教师科研启动项目，2023-04至2028-04，20万元，在研，主持。

4. 国家自然科学基金委员会, 面上项目, 22078097, ω-转氨酶双底物识别的分子基础及其理性改造, 2021-01-01 至 2024-12-31, 63万元, 在研, 参与。

5. 国家自然科学基金委员会, 地区科学基金项目, 31760449, 曲霉型豆豉发酵微生物优势菌群分析及代谢机理研究, 2018-01-01 至 2021-12-31, 38万元, 结题, 参与。

五、论文和专利

  期刊论文：

[1] Yang Lin, Zhang Kaiyue, Xu Meng, Xie Youyu, Meng Xiangqi, Wang Hualei*, Wei Dongzhi*. Mechanism‐Guided Computational Design of ω‐Transaminase by Reprograming of High‐Energy‐Barrier Steps[J]. Angewandte Chemie-International Edition, 2022, 61(52): e202212555. [IF=16.8]

[2] Zhong Minghui, Yang Lin (co-first author), Xiong Kai, Yang Huilin, Wang Xiaolan*. Exploring the Mechanism of Self-Consistent Balance between Microbiota and High Efficiency in Wastewater Treatment[J]. Bioresource Technology, 2023, 374: 128785. [IF=11.9]

[3] Yang Lin, Zhang Yiping, Liu Yan, Wang Hualei*, Wei Dongzhi. Highly efficient synthesis of pharmaceutically relevant chiral 3-N-substituted-azacyclic alcohols using two enantiocomplementary short chain dehydrogenases[J]. Biochemical Engineering Journal, 2022, 178: 108300. [IF=4.4]

[4] Liu Yan, Zhong Xuezhao, Luo Zi, Meng Xiangqi, Li Rui, Zhong Wa, Yang Lin, Wang Hualei*, Wei Dongzhi. The identification of a robust leucine dehydrogenase from a directed soil metagenome for efficient synthesis of L‐2‐aminobutyric acid[J]. Biotechnology Journal, 2023: 2200590. [IF=5.7]

[5] Meng Xiangqi, Liu Yan, Yang Lin, Li Rui, Wang Hualei*, Shen Yalin, Wei Dongzhi. Rational identification of a high catalytic efficiency leucine dehydrogenase and process development for efficient synthesis of L-phenylglycine[J]. Biotechnology Journal, 2023: 2200465. [IF=5.7]

[6] Ye Wenjie, Xie Jingwen, Liu Yan, Wang Yilin, Zhang Xinyu, Yang Xiaoying, Yang Lin, Wang Hualei*, Wei Dongzhi. Enhancing the Activity of an Alcohol Dehydrogenase by Using “Aromatic Residue Scanning” at Potential Plasticity Sites[J]. Chemistry–A European Journal, 2023: e202203530. [IF=5.0]

[7] Ye Wenjie, Zhang Yuxin, Wang Yilin, Xie Jingwen, Liu Yan, Yang Lin, Wang Hualei*, Wei Dongzhi. Engineering a Medium‐chain Alcohol Dehydrogenase for Efficient Synthesis of (S)‐N‐Boc‐3‐pyrrolidinol by Adjusting the Conformational Dynamics of Loops[J]. ChemCatChem, 2023, 15: e2022011. [IF=5.5]

[8] Chen Zhi*, Xu Xiangjing, Ju Xin, Yan Lishi, Li Liangzhi, Yang Lin. Sources, Components, Structure, Catalytic Mechanism and Applications: a Critical Review on Nicotinate Dehydrogenase (NDHase)[J]. Journal of Microbiology and Biotechnology, 2023, 33(6): 1-8. [IF=2.8]

[9] Xie Youyu, Wang Jiguo, Yang Lin, Wang Wei, Liu Qinghai, Wang Hualei*, Wei Dongzhi*. The identification and application of a robust ω-transaminase with high tolerance towards substrates and isopropylamine from a directed soil metagenome[J]. Catalysis Science & Technology, 2022, 12(7): 2162-2175. [IF=6.2]

[10] Meng Xiangqi, Yang Lin, Liu Yan, Wang Hualei*, Shen Yalin, Wei Dongzhi*. Identification and rational engineering of a high substrate-tolerant leucine dehydrogenase effective for synthesis of L-tert-leucine[J]. ChemCatChem, 2021, 13(14): 3340-3349. [IF=5.5]

[11] Chen Zhi, Wang Hualei*, Yang Lin, Jiang Shuiqing, Wei Dongzhi*. Significantly enhancing the stereoselectivity of a regioselective nitrilase for the production of (S)-3-cyano-5-methylhexanoic acid using an MM/PBSA method[J]. Chemical Communications, 2021, 57(7): 931-934. [IF=6.1]

[12] Xie Youyu, Xu Feng, Yang Lin, Liu He, Xu Xiangyang, Wang Hualei*, Wei Dongzhi*. Engineering the large pocket of an (S)-selective transaminase for asymmetric synthesis of (S)-1-amino-1-phenylpropane[J]. Catalysis Science & Technology, 2021, 11(7): 2461-2470. [IF=6.2]

  论著之外的代表性研究成果和学术奖励：

[1] 王筱兰; 李保根; 涂宗财; 杨林; 杨慧林; 一株分离自豆豉中产蛋白酶的解淀粉芽孢杆菌菌株, 2017- 12-1, 中国, ZL201510073251.7(专利)

[2] 王白杨; 王筱兰; 杨林; 一种高盐耐受环境下的耐盐菌菌株及其筛选方法和应用, 2016-7-13, 中国, ZL201610547861.0 (专利)

六、联系方式

地址：生命学院3420实验室

Email:1012835610@qq.com